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Addgene

pClneoFLAG human RASSF4
(Plasmid #37020)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37020 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCl-neo (modified)
  • Backbone manufacturer
    Promega
  • Modifications to backbone
    pCI-neo vector was modified to include FLAG tag N-terminal to MCS.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    rassf4
  • Species
    H. sapiens (human)
  • GenBank ID
    BC032593
  • Entrez Gene
    RASSF4 (a.k.a. AD037)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    cDNA encoding human RASSF4 (BC032593) was purchased from Open Biosystems

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene's sequencing results indicate there are two EcoRI sites after FLAG

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pClneoFLAG human RASSF4 was a gift from Yutaka Hata (Addgene plasmid # 37020 ; http://n2t.net/addgene:37020 ; RRID:Addgene_37020)

  • For your References section:

    Hippo pathway-dependent and -independent roles of RASSF6. Ikeda M, Kawata A, Nishikawa M, Tateishi Y, Yamaguchi M, Nakagawa K, Hirabayashi S, Bao Y, Hidaka S, Hirata Y, Hata Y. Sci Signal. 2009 Sep 29;2(90):ra59. 10.1126/scisignal.2000300 PubMed 19797269
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