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pClneoFH human RASSF3
(Plasmid #37019)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37019 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCl-neo (modified)
  • Backbone manufacturer
    Promega
  • Modifications to backbone
    pCI-neo vector was modified to include FLAG and His tags N-terminal to MCS. The resulting vector was named pCIneoFLAG-His6(FH).
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RASSF3
  • Species
    H. sapiens (human)
  • GenBank ID
    BC055023
  • Entrez Gene
    RASSF3 (a.k.a. RASSF5)
  • Promoter CMV
  • Tags / Fusion Proteins
    • FLAG (N terminal on backbone)
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    cDNAs encoding human RASSF3 (BC055023)was purchased from Open Biosystems

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pCIneoFH vector has the sequence NheI-FLAG-His6-EcoRI-MluI-XbaI-SalI-SmaI-NotI.

A digest with NheI and SalI will excise the insert with tags.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pClneoFH human RASSF3 was a gift from Yutaka Hata (Addgene plasmid # 37019 ; http://n2t.net/addgene:37019 ; RRID:Addgene_37019)

  • For your References section:

    Hippo pathway-dependent and -independent roles of RASSF6. Ikeda M, Kawata A, Nishikawa M, Tateishi Y, Yamaguchi M, Nakagawa K, Hirabayashi S, Bao Y, Hidaka S, Hirata Y, Hata Y. Sci Signal. 2009 Sep 29;2(90):ra59. 10.1126/scisignal.2000300 PubMed 19797269
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