pcDNA3-SypHluorin 4x (S4x)
(Plasmid #37005)

• Depositing Labs: Stephen Heinemann, Yongling Zhu
• Publication: Zhu et al Neuron. 2009 Feb 12;61(3):397-411.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SypHluorin 4x
• Species: R. norvegicus (rat)
• Mutation: Four copies of pHluorin inserted at site 184-185
• Entrez Gene: Syp (a.k.a. Syp1)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV fwd
• 3′ sequencing primer: BGH-rev

Resource Information

• Supplemental Documents:
  • pcDNA3-SypHluorin4x_seq.pdf
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry