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Purpose(Empty Backbone)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 36999 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBABE-Puro
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Backbone manufacturerAddgene plasmid #1764
- Backbone size (bp) 4517
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Modifications to backbonePuro was replaced with EGFP using HindIII and ClaI
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)Stbl3
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pBABE_5 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBABE-EGFP was a gift from Debu Chakravarti (Addgene plasmid # 36999 ; http://n2t.net/addgene:36999 ; RRID:Addgene_36999) -
For your References section:
A Transcriptional Regulatory Role of the THAP11-HCF-1 Complex in Colon Cancer Cell Function. Parker JB, Palchaudhuri S, Yin H, Wei J, Chakravarti D. Mol Cell Biol. 2012 May;32(9):1654-70. Epub 2012 Feb 27. 10.1128/MCB.06033-11 PubMed 22371484