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Addgene

pBABE-puro-THAP11-rescue-3xFLAG
(Plasmid #36998)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 36998 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBABE-Puro
  • Backbone manufacturer
    Addgene plasmid #1764
  • Backbone size w/o insert (bp) 5169
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    THAP11
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1029
  • Mutation
    Mutations were generated at codons 273-275 converting GAA GTG AAG to GAG GTC AAA and at codons 295-297 converting GAA CTG CGT to GAG CTC CGC
  • Entrez Gene
    THAP11 (a.k.a. HRIHFB2206, CTG-B43a, CTG-B45d, RONIN)
  • Tag / Fusion Protein
    • 3xFLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer pBABE_5
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

3xFLAG-THAP11 was subcloned from Addgene plasmids #37001

Mutations were generated at codons 273-275 converting GAA GTG AAG to GAG GTC AAA
and at codons 295-297 converting GAA CTG CGT to GAG CTC CGC

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABE-puro-THAP11-rescue-3xFLAG was a gift from Debu Chakravarti (Addgene plasmid # 36998 ; http://n2t.net/addgene:36998 ; RRID:Addgene_36998)

  • For your References section:

    A Transcriptional Regulatory Role of the THAP11-HCF-1 Complex in Colon Cancer Cell Function. Parker JB, Palchaudhuri S, Yin H, Wei J, Chakravarti D. Mol Cell Biol. 2012 May;32(9):1654-70. Epub 2012 Feb 27. 10.1128/MCB.06033-11 PubMed 22371484
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