Desmocollin2_tail-GST
(Plasmid
#36992)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 36992 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonePGEX4T-1
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Backbone manufacturerGE Healthcare
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDsc2
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Alt nameDesmocollin2
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SpeciesH. sapiens (human)
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MutationIntracellular domain of Dsc2 (nt 2,603–3,158)
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GenBank IDNM_024422.3
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Entrez GeneDSC2 (a.k.a. ARVD11, CDHF2, DG2, DGII/III, DSC3)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer pGEX_5 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Desmocollin2_tail-GST was a gift from Kathleen Green (Addgene plasmid # 36992 ; http://n2t.net/addgene:36992 ; RRID:Addgene_36992) -
For your References section:
Desmosomal cadherins utilize distinct kinesins for assembly into desmosomes. Nekrasova OE, Amargo EV, Smith WO, Chen J, Kreitzer GE, Green KJ. J Cell Biol. 2011 Dec 26;195(7):1185-203. Epub 2011 Dec 19. 10.1083/jcb.201106057 PubMed 22184201