Desmoglein2_tail-GST
(Plasmid
#36990)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 36990 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonePGEX4T-1
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Backbone manufacturerGE Healthcare
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDsg2
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Alt nameDesmoglein2
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SpeciesH. sapiens (human)
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MutationIntracellular domain of DSG2 (nucleotides 1911–3516)
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Entrez GeneDSG2 (a.k.a. CDHF5, HDGC)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer pGEX_5 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Desmoglein2_tail-GST was a gift from Kathleen Green (Addgene plasmid # 36990 ; http://n2t.net/addgene:36990 ; RRID:Addgene_36990) -
For your References section:
A role for caveolin-1 in desmoglein binding and desmosome dynamics. Brennan D, Peltonen S, Dowling A, Medhat W, Green KJ, Wahl JK 3rd, Del Galdo F, Mahoney MG. Oncogene. 2012 Mar 29;31(13):1636-48. doi: 10.1038/onc.2011.346. Epub 2011 Aug 15. 10.1038/onc.2011.346 PubMed 21841821