1105-Desmoglein2-FLAG-eGFP
(Plasmid #36989)

• Depositing Lab: Kathleen Green
• Publication: Klessner et al Mol Biol Cell. 2009 Jan;20(1):328-37. Epub 2008 Nov 5.

Backbone

• Vector backbone: pEGFP-N2
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Dsg2
• Alt name: Desmoglein2
• Species: H. sapiens (human)
• Entrez Gene: DSG2 (a.k.a. CDHF5, HDGC)
• Promoter: CMV
• Tags / Fusion Proteins:
  • FLAG (C terminal on insert)
  • eGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: EGFP-N

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The construct encoding human Dsg2 cDNA in pBluescript was a gift from Drs. W. Franke and S. Schafer, German Cancer Research Center, Heidelberg, Germany.