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Addgene

pCMMP-BiP-IRES-mRFP
(Plasmid #36975)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 36975 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCMMP-MCS-IRES-mRFP
  • Backbone manufacturer
    Addgene plasmid #36972
  • Total vector size (bp) 9031
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    BiP
  • Alt name
    heat shock 70-kDa protein 5
  • Species
    H. sapiens (human)
  • Entrez Gene
    HSPA5 (a.k.a. BIP, GRP78, HEL-S-89n)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MfeI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer pLXSN_5
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Sugden lab plasmid p3857

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMMP-BiP-IRES-mRFP was a gift from Bill Sugden (Addgene plasmid # 36975 ; http://n2t.net/addgene:36975 ; RRID:Addgene_36975)
  • For your References section:

    The Latent Membrane Protein 1 (LMP1) Oncogene of Epstein-Barr Virus Can Simultaneously Induce and Inhibit Apoptosis in B Cells. Pratt ZL, Zhang J, Sugden B. J Virol. 2012 Apr;86(8):4380-93. Epub 2012 Feb 8. 10.1128/JVI.06966-11 PubMed 22318153