Skip to main content
Addgene

pCMMP-NGFR:LMP1-IRES-mRFP
(Plasmid #36974)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 36974 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCMMP-MCS-IRES-mRFP
  • Backbone manufacturer
    Addgene plasmid #36972
  • Total vector size (bp) 8596
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NGFR:LMP1
  • Species
    H. sapiens (human)
  • Mutation
    NGFR:LMP1 chimeric receptor consists of aa 1–276 of NGF-R (extracellular and transmembrane domain) fused to aa 192–386 of LMP1 (cytoplasmic domain)
  • Entrez Gene
    NGFR (a.k.a. CD271, Gp80-LNGFR, TNFRSF16, p75(NTR), p75NTR)
  • Entrez Gene
    LMP-1 (a.k.a. HHV4_LMP-1)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NA (unknown if destroyed)
  • 3′ cloning site NA (unknown if destroyed)
  • 5′ sequencing primer pLXSN_5
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Sugden lab plasmid p3856

The NGFR-LMP1 fusion in the plasmid contains the amino terminus of NGFR fused to the carboxy terminus of LMP1. The carboxy-terminal signaling of LMP1 is conditionally activated when anti-NGFR antibody crosslinks with the NGFR
moieties. Please see associated publication (link below) for more details.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMMP-NGFR:LMP1-IRES-mRFP was a gift from Bill Sugden (Addgene plasmid # 36974 ; http://n2t.net/addgene:36974 ; RRID:Addgene_36974)

  • For your References section:

    The Latent Membrane Protein 1 (LMP1) Oncogene of Epstein-Barr Virus Can Simultaneously Induce and Inhibit Apoptosis in B Cells. Pratt ZL, Zhang J, Sugden B. J Virol. 2012 Apr;86(8):4380-93. Epub 2012 Feb 8. 10.1128/JVI.06966-11 PubMed 22318153
Related items:
Commonly requested with: