NC16 pCDNA3.1 FLAG NRF2
(Plasmid #36971)

• Depositing Lab: Randall Moon
• Publication: Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50. Epub 2012 Jan 3.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NRF2
• Species: H. sapiens (human)
• Insert Size (bp): 1815
• Entrez Gene: NFE2L2 (a.k.a. HEBP1, IMDDHH, NRF2, Nrf-2)
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AscI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH rev

Resource Information

• Articles Citing this Plasmid:
  • 26 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry