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Addgene

NC15 pGLUE CUL3
(Plasmid #36970)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 36970 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGLUE
  • Backbone manufacturer
    Moon Lab Plasmid available from Addgene (plasmid #15100)
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CUL3
  • Alt name
    cullin 3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2400
  • Entrez Gene
    CUL3 (a.k.a. CUL-3, NEDAUS, PHA2E)
  • Promoter CMV
  • Tags / Fusion Proteins
    • streptag (N terminal on backbone)
    • HA (N terminal on backbone)
    • CBP tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Learn more about the pGLUE tandem-affinity purification plasmid backbone at http://www.addgene.org/15100/ .

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NC15 pGLUE CUL3 was a gift from Randall Moon (Addgene plasmid # 36970 ; http://n2t.net/addgene:36970 ; RRID:Addgene_36970)

  • For your References section:

    Wilms tumor gene on X chromosome (WTX) inhibits degradation of NRF2 protein through competitive binding to KEAP1 protein. Camp ND, James RG, Dawson DW, Yan F, Davison JM, Houck SA, Tang X, Zheng N, Major MB, Moon RT. J Biol Chem. 2012 Feb 24;287(9):6539-50. Epub 2012 Jan 3. 10.1074/jbc.M111.316471 PubMed 22215675
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