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Addgene

NC8 pCDNA3.1 VENUS WTX S286D
(Plasmid #36963)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 36963 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1 (with Venus added)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 6100
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    WTX S286D
  • Alt name
    Wilms tumor gene on the X chromosome
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2400
  • Mutation
    S286D
  • Entrez Gene
    AMER1 (a.k.a. FAM123B, OSCS, WTX)
  • Promoter CMV
  • Tag / Fusion Protein
    • Venus (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer EGFP C
  • 3′ sequencing primer BGH rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

T798I mutation found in Addgene's QC sequencing should not alter the function of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NC8 pCDNA3.1 VENUS WTX S286D was a gift from Randall Moon (Addgene plasmid # 36963 ; http://n2t.net/addgene:36963 ; RRID:Addgene_36963)

  • For your References section:

    Wilms tumor gene on X chromosome (WTX) inhibits degradation of NRF2 protein through competitive binding to KEAP1 protein. Camp ND, James RG, Dawson DW, Yan F, Davison JM, Houck SA, Tang X, Zheng N, Major MB, Moon RT. J Biol Chem. 2012 Feb 24;287(9):6539-50. Epub 2012 Jan 3. 10.1074/jbc.M111.316471 PubMed 22215675
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