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Addgene

NC3 pCDNA3.1 FLAG WTX (1-804)
(Plasmid #36958)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 36958 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    WTX
  • Alt name
    Wilms tumor gene on the X chromosome
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2400
  • Entrez Gene
    AMER1 (a.k.a. FAM123B, OSCS, WTX)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NC3 pCDNA3.1 FLAG WTX (1-804) was a gift from Randall Moon (Addgene plasmid # 36958 ; http://n2t.net/addgene:36958 ; RRID:Addgene_36958)

  • For your References section:

    Wilms tumor suppressor WTX negatively regulates WNT/beta-catenin signaling. Major MB, Camp ND, Berndt JD, Yi X, Goldenberg SJ, Hubbert C, Biechele TL, Gingras AC, Zheng N, Maccoss MJ, Angers S, Moon RT. Science. 2007 May 18;316(5827):1043-6. 10.1126/science/1141515 PubMed 17510365
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