pCMMP-LMP1-IRES-eGFP
(Plasmid #36955)

• Depositing Lab: Bill Sugden
• Publication: Pratt et al J Virol. 2012 Apr;86(8):4380-93. Epub 2012 Feb 8.

Backbone

• Vector backbone: pCMMP-MCS-IRES-eGFP
• Backbone manufacturer: Addgene plasmid #36953
• Backbone size w/o insert (bp): 7037
• Total vector size (bp): 8495
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: LMP1
• Alt name: latent membrane protein 1 (LMP1) of Epstein-Barr virus (EBV)
• Entrez Gene: LMP-1 (a.k.a. HHV4_LMP-1)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NA (unknown if destroyed)
• 3′ cloning site: NA (unknown if destroyed)
• 5′ sequencing primer: pLXSN_5

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Sugden lab plasmid p3455

Please note that the full plasmid sequence provided by the depositing laboratory and Addgene's sequencing results differ from GenBank entry YP_401722.1 by three polymorphisms: F106Y, I122L and M129I. These differences result from the particular EBV isolate. For more information please see the following article: https://www.ncbi.nlm.nih.gov/pubmed/7931160