pCMMP-dnPERK-IRES-eGFP
(Plasmid #36954)

• Depositing Lab: Bill Sugden
• Publication: Lee et al Blood. 2008 Feb 15;111(4):2280-9. Epub 2007 Nov 27.

Backbone

• Vector backbone: pCMMP-MCS-IRES-eGFP
• Backbone manufacturer: Addgene plasmid #36953
• Backbone size w/o insert (bp): 7037
• Total vector size (bp): 10074
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: dnPERK
• Alt name: PKR-like ER kinase
• Species: H. sapiens (human)
• Mutation: deletion of aa592-1061 to create dominant negative
• Entrez Gene: EIF2AK3 (a.k.a. PEK, PERK, WRS)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NA (unknown if destroyed)
• 3′ cloning site: NA (unknown if destroyed)
• 5′ sequencing primer: pLXSN_5

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Sugden lab plasmid p3236

dnPERK from Dr Ron Wek, Indiana University School of Medicine, Indianapolis.

When compared to the GenBank reference sequence, the full plasmid sequence indicates that the PERK insert contains the following polymorphisms: a deletion of amino acid L21 and Q166R and N490H mutations.