pET16b-Trx C35S
(Plasmid
#36947)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 36947 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET16b
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5711
- Total vector size (bp) 6000
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Modifications to backbonenone
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsExpression of protein in BL21 cells and purification by His tag
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameThioredoxin-1 C35S mutation
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Alt nameTrx-1
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Alt nameTrx
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SpeciesH. sapiens (human)
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Insert Size (bp)318
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Mutationcys 35 to serine
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GenBank IDNM_003329
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Entrez GeneTXN (a.k.a. TRDX, TRX, TRX1, TXN1, Trx80)
- Promoter T7
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Tag
/ Fusion Protein
- His tag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byOriginal unmutated gene obtained from Origene
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET16b-Trx C35S was a gift from Anna Blom (Addgene plasmid # 36947 ; http://n2t.net/addgene:36947 ; RRID:Addgene_36947) -
For your References section:
Truncated and full-length thioredoxin-1 have opposing activating and inhibitory properties for human complement with relevance to endothelial surfaces. King BC, Nowakowska J, Karsten CM, Kohl J, Renstrom E, Blom AM. J Immunol. 2012 Apr 15;188(8):4103-12. Epub 2012 Mar 19. 10.4049/jimmunol.1101295 PubMed 22430737