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pET16b-Trx CC32/35SS
(Plasmid #36946)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 36946 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET16b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5711
  • Total vector size (bp) 6000
  • Modifications to backbone
    none
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Expression of protein in BL21 cells and purification by His tag
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Thioredoxin 1
  • Alt name
    Trx-1
  • Alt name
    Trx
  • Alt name
    TXN
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    318
  • Mutation
    both active site cysteines (32,35) mutated to serine
  • GenBank ID
    NM_003329
  • Entrez Gene
    TXN (a.k.a. TRDX, TRX, TRX1, TXN1, Trx80)
  • Promoter T7
  • Tag / Fusion Protein
    • His tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Original unmutated gene obtained from Origene

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET16b-Trx CC32/35SS was a gift from Anna Blom (Addgene plasmid # 36946 ; http://n2t.net/addgene:36946 ; RRID:Addgene_36946)

  • For your References section:

    Truncated and full-length thioredoxin-1 have opposing activating and inhibitory properties for human complement with relevance to endothelial surfaces. King BC, Nowakowska J, Karsten CM, Kohl J, Renstrom E, Blom AM. J Immunol. 2012 Apr 15;188(8):4103-12. Epub 2012 Mar 19. 10.4049/jimmunol.1101295 PubMed 22430737
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