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pET16b-Trx80
(Plasmid #36945)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 36945 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET16b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5711
  • Total vector size (bp) 6000
  • Modifications to backbone
    none
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Expression of protein in BL21 cells and purification by His tag: unlike the full-length Trx proteins, this truncated form is expressed in inclusion bodies. Truncated protein is formed in vivo by cleavage of the full-length Trx1.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Trx80 (truncated Trx)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    317
  • Mutation
    premature stop inserted after K80 in Trx1 gene
  • GenBank ID
    NM_003329
  • Entrez Gene
    TXN (a.k.a. TRDX, TRX, TRX1, TXN1, Trx80)
  • Promoter T7
  • Tag / Fusion Protein
    • His tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Original unmutated gene obtained from Origene

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

identification of Trx80: J Biol Chem. 2000 Dec 1;275(48):37474-80.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET16b-Trx80 was a gift from Anna Blom (Addgene plasmid # 36945 ; http://n2t.net/addgene:36945 ; RRID:Addgene_36945)

  • For your References section:

    Truncated and full-length thioredoxin-1 have opposing activating and inhibitory properties for human complement with relevance to endothelial surfaces. King BC, Nowakowska J, Karsten CM, Kohl J, Renstrom E, Blom AM. J Immunol. 2012 Apr 15;188(8):4103-12. Epub 2012 Mar 19. 10.4049/jimmunol.1101295 PubMed 22430737
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