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Purpose(Empty Backbone) SGC Empty backbone for bacterial expression
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 36943 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepET28-SBP-TEV
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Backbone manufacturerSGC
- Backbone size (bp) 7445
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Vector typeBacterial Expression
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Tag
/ Fusion Protein
- 6xHistag-SBP-TEV (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Cloning Information
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28-SBP-TEV was a gift from Cheryl Arrowsmith (Addgene plasmid # 36943 ; http://n2t.net/addgene:36943 ; RRID:Addgene_36943)
Map uploaded by Addgene staff.
