NHA-Delta 5b
(Plasmid #36937)

• Depositing Lab: Edward Chan
• Publication: Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-47. Epub 2010 Dec 2.

Backbone

• Vector backbone: pCl-neo_NHA
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 6259
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TNRC6A Delta 5b
• Alt name: Delta 5b
• Species: H. sapiens (human)
• Insert Size (bp): 876
• Mutation: aa 1660-1912 only
• Entrez Gene: TNRC6A (a.k.a. CAGH26, FAME6, GW1, GW182, TNRC6)
• Tag / Fusion Protein:
  • NHA (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7
• 3′ sequencing primer: EBV-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

L->P mutation detected in Addgene QC sequence. This mutation is not known to affect function.