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Addgene

NHA-Delta 12a
(Plasmid #36933)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 36933 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCl-neo_NHA
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 5950
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TNRC6A Delta 12a
  • Alt name
    Delta 12a
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    969
  • Mutation
    aa 896-1045 only
  • Entrez Gene
    TNRC6A (a.k.a. CAGH26, FAME6, GW1, GW182, TNRC6)
  • Tag / Fusion Protein
    • NHA (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NHA-Delta 12a was a gift from Edward Chan (Addgene plasmid # 36933 ; http://n2t.net/addgene:36933 ; RRID:Addgene_36933)

  • For your References section:

    Divergent GW182 functional domains in the regulation of translational silencing. Yao B, Li S, Jung HM, Lian SL, Abadal GX, Han F, Fritzler MJ, Chan EK. Nucleic Acids Res. 2011 Apr;39(7):2534-47. Epub 2010 Dec 2. 10.1093/nar/gkq1099 PubMed 21131274
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