NHA-Delta 10
(Plasmid #36925)

• Depositing Lab: Edward Chan
• Publication: Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-47. Epub 2010 Dec 2.

Backbone

• Vector backbone: pCl-neo_NHA
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 7567
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TNGW1
• Alt name: Delta 10
• Species: H. sapiens (human)
• Insert Size (bp): 2067
• Mutation: aa 566-1343 only
• GenBank ID: KIAA1460
• Tag / Fusion Protein:
  • NHA (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: EBV-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There is a typo in the NAR paper that incorrectly lists the N-term truncation as AA655 (instead of the correct AA566).