NHA-Ago2
(Plasmid
#36922)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 36922 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCl-neo_NHA
- Backbone size w/o insert (bp) 5500
- Total vector size (bp) 8080
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAgo2
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SpeciesH. sapiens (human)
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Insert Size (bp)2580
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Entrez GeneAGO2 (a.k.a. CASC7, EIF2C2, LESKRES, LINC00980, PPD, Q10)
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Tag
/ Fusion Protein
- NHA (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byDerived from Tom Hobman’s clone. Their sequence has a different N-terminal peptide than others used in the literature.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
NHA-Ago2 was a gift from Edward Chan (Addgene plasmid # 36922 ; http://n2t.net/addgene:36922 ; RRID:Addgene_36922) -
For your References section:
Divergent GW182 functional domains in the regulation of translational silencing. Yao B, Li S, Jung HM, Lian SL, Abadal GX, Han F, Fritzler MJ, Chan EK. Nucleic Acids Res. 2011 Apr;39(7):2534-47. Epub 2010 Dec 2. 10.1093/nar/gkq1099 PubMed 21131274