pRLG3A
(Plasmid #36921)

• Depositing Lab: Manuela Martins-Green
• Publication: Zheng et al Transgenic Res. 2008 Aug;17(4):741-7. Epub 2008 Jan 9.

Backbone

• Vector backbone: pCAGGS
• Backbone size w/o insert (bp): 4802
• Total vector size (bp): 4802
• Modifications to backbone: Please see associated publication
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: red fluorescent protein
• Alt name: RFP
• Insert Size (bp): 750
• GenBank ID:

Gene/Insert 2

• Gene/Insert name: eGFP
• Alt name: GFP
• Insert Size (bp): 750
• GenBank ID:

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This vector contains a MCS and combines the Cre/loxP with a bicistronic transcript that contains dual fluorescent and bioluminescent cell markers. mRFP was used as a marker for easy genotyping and tissue labeling. A chimeric membrane-localizing luciferase/eGFP fusion protein was designed as a secondary marker, which upon Cre activation, highlights the cells expressing the transgene with eGFP as well as luciferase activity.