PaBphP 1-497
(Plasmid
#36920)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 36920 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28a
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5369
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePaBphP-PCD
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SpeciesP. aeruginosa
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Insert Size (bp)1491
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MutationContains the photosensory core domain coding region (residues 1–497)
- Promoter T7
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Tag
/ Fusion Protein
- His (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PaBphP 1-497 was a gift from Keith Moffat (Addgene plasmid # 36920 ; http://n2t.net/addgene:36920 ; RRID:Addgene_36920) -
For your References section:
Temperature-scan cryocrystallography reveals reaction intermediates in bacteriophytochrome. Yang X, Ren Z, Kuk J, Moffat K. Nature. 2011 Oct 16;479(7373):428-32. doi: 10.1038/nature10506. 10.1038/nature10506 PubMed 22002602