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Addgene

GST-beta-arrestin2
(Plasmid #36919)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 36919 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-4T1
  • Backbone size w/o insert (bp) 4969
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    beta-arrestin2
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Arrb2 (a.k.a. BARRES)
  • Tag / Fusion Protein
    • GST (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer pGEX5'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GST-beta-arrestin2 was a gift from Robert Lefkowitz (Addgene plasmid # 36919 ; http://n2t.net/addgene:36919 ; RRID:Addgene_36919)

  • For your References section:

    Activation-dependent conformational changes in {beta}-arrestin 2. Xiao K, Shenoy SK, Nobles K, Lefkowitz RJ. J Biol Chem. 2004 Dec 31;279(53):55744-53. Epub 2004 Oct 22. 10.1074/jbc.M409785200 PubMed 15501822
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