beta-arrestin2 mYFP
(Plasmid #36917)

• Depositing Lab: Robert Lefkowitz
• Publication: Violin et al J Biol Chem. 2006 Jul 21;281(29):20577-88. Epub 2006 May 10.

Backbone

• Vector backbone: pcDNA3.1 zeo
• Backbone size w/o insert (bp): 5015
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: beta-arrestin2
• Species: R. norvegicus (rat)
• Insert Size (bp): 1900
• Entrez Gene: Arrb2 (a.k.a. BARRES)
• Promoter: CMV
• Tag / Fusion Protein:
  • mYFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (unknown if destroyed)
• 3′ cloning site: XbaI (unknown if destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry