pCA-T-intron(Neo)-G(LNL)
(Plasmid #36908)

• Depositing Lab: Liqun Luo
• Publication: Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Mar 27.

Backbone

• Vector backbone: pBluescript SK(+)
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3000
• Total vector size (bp): 7412
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: T (i.e., ATG, start codon as a first exon)
• Promoter: CAG (chicken beta actin promoter and CMV enhancer)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: see sequence
• 3′ sequencing primer: see sequence

Gene/Insert 2

• Gene/Insert name: GFP
• Alt name: mut4EGFP
• Mutation: deleted nucleotides before nucleotide 275
• Promoter: CAG (chicken beta actin promoter and CMV enhancer)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: see sequence
• 3′ sequencing primer: T3

Gene/Insert 3

• Gene/Insert name: beta-globin intron
• Mutation: insertion of loxP-Neo-loxP
• Promoter: CAG (chicken beta actin promoter and CMV enhancer)

Cloning Information for Gene/Insert 3

• Cloning method: Unknown
• 5′ sequencing primer: see sequence
• 3′ sequencing primer: see sequence

Gene/Insert 4

• Gene/Insert name: Neo
• Alt name: neomycin phosphotransferase
• Alt name: neomycin resistance gene
• Promoter: SV40

Cloning Information for Gene/Insert 4

• Cloning method: Unknown
• 5′ sequencing primer: see sequence
• 3′ sequencing primer: see sequence

Resource Information

• Supplemental Documents:
  • pCA-T-intron(Neo)-G(LNL)_GenBank_file
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is the 'LNL' version of the plasmid. It contains loxP-Neo-loxP in the intron. The 'FLLFLNL' version (loxP-Neo-loxP) is also available from Addgene.