ANKRA2
(Plasmid
#36904)
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PurposeBacterial expression for structure determination; may not be full ORF
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 36904 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepET28-MHL (Addgene plasmid 26096)
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Backbone manufacturerSGC
- Backbone size w/o insert (bp) 7325
- Total vector size (bp) 7823
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameANKRA2
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Alt namePDB: 3V2O, 3V2X, 3V31
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Alt nameankyrin repeat, family A (RFXANK-like) 2
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SpeciesH. sapiens (human)
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Insert Size (bp)498
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Mutationamino acid residues 148-313 (ANK repeats)
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GenBank IDBC012917
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Entrez GeneANKRA2 (a.k.a. ANKRA)
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Tag
/ Fusion Protein
- His tag with TEV cleavage (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
PDB: 3V2O. http://www.thesgc.org/structures/3V2O/
PDB: 3V2X http://www.thesgc.org/structures/3V2X/
PDB: 3V31 http://www.thesgc.org/structures/3V31/
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ANKRA2 was a gift from Cheryl Arrowsmith (Addgene plasmid # 36904 ; http://n2t.net/addgene:36904 ; RRID:Addgene_36904)