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Addgene

YWHAG
(Plasmid #36903)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 36903 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pTvHR21-SGC
  • Backbone manufacturer
    SGC Oxford
  • Backbone size w/o insert (bp) 7387
  • Total vector size (bp) 8114
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    YWHAG
  • Alt name
    PDB: 3UZD, 4O46
  • Alt name
    tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide (14-3-3gamma)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    786
  • Entrez Gene
    YWHAG (a.k.a. 14-3-3GAMMA, DEE56, EIEE56, PPP1R170)
  • Tag / Fusion Protein
    • 6xHis with TEV Cleavage (C terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer unknown
  • 3′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    YWHAG was a gift from Cheryl Arrowsmith (Addgene plasmid # 36903 ; http://n2t.net/addgene:36903 ; RRID:Addgene_36903)
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