pMADM-beta
(Plasmid #36891)

• Depositing Lab: Liqun Luo
• Publication: Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Mar 27.

Backbone

• Vector backbone: pBluescript SK(+)
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3000
• Total vector size (bp): 14025
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418) ; thymidine kinase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Beta-Geo
• Alt name: neomycin phosphotransferase and beta-galactosidase fusion
• Promoter: CAG (chicken beta actin promoter and CMV enhancer)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: see sequence
• 3′ sequencing primer: see sequence

Gene/Insert 2

• Gene/Insert name: thymidine kinase
• Promoter: PGK (phosphoglycerate kinase) promoter

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: see sequence
• 3′ sequencing primer: see sequence

Resource Information

• Supplemental Documents:
  • pMADM-beta_GenBank_file

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry