pBT268_(pCA-ATG-intron-tTA2-iiTRE-tdT3Mycii)
(Plasmid #36880)

• Depositing Lab: Liqun Luo
• Publication: Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Mar 27.

Backbone

• Vector backbone: pBluescript SK(+)
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3000
• Total vector size (bp): 9142
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl2
• Growth instructions: If plasmid yields are poor in DH5alpha, we recommend Stbl2 cells (Invitrogen) grown at 30C.
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: tTA2
• Alt name: tetracycline transactivator 2
• Mutation: insertion of a beta-globin intron with a loxP site after start codon of tTA2
• Promoter: CAG (chicken beta actin promoter and CMV enhancer)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: see sequence
• 3′ sequencing primer: see sequence

Gene/Insert 2

• Gene/Insert name: tdT-3Myc
• Alt name: tdTomato
• Promoter: TRE (tetO)
• Tag / Fusion Protein:
  • 3 Myc tags (C terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: see sequence
• 3′ sequencing primer: see sequence

Gene/Insert 3

• Gene/Insert name: insulator
• Alt name: chicken beta-globin HS4 insulator core (~250 bp)
• Promoter: none

Cloning Information for Gene/Insert 3

• Cloning method: Unknown
• 5′ sequencing primer: see sequence
• 3′ sequencing primer: see sequence

Resource Information

• Supplemental Documents:
  • pBT268_GenBank_file
• A portion of this plasmid was derived from a plasmid made by: W. Hillen and R. Tsien
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry