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Addgene

pBT267_(pCA-ATG-intron-tTA2-TRE-tdT3Myc)
(Plasmid #36879)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 36879 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBluescript SK(+)
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 8161
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    tTA2
  • Alt name
    tetracycline transactivator 2
  • Mutation
    insertion of a beta-globin intron with a loxP site after start codon of tTA2
  • Promoter CAG (chicken beta actin promoter and CMV enhancer)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    tdT-3Myc
  • Alt name
    tdTomato
  • Promoter TRE (tetO)
  • Tag / Fusion Protein
    • 3 Myc tags (C terminal on insert)

Cloning Information for Gene/Insert 2

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    W. Hillen

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBT267_(pCA-ATG-intron-tTA2-TRE-tdT3Myc) was a gift from Liqun Luo (Addgene plasmid # 36879 ; http://n2t.net/addgene:36879 ; RRID:Addgene_36879)
  • For your References section:

    Extensions of MADM (Mosaic Analysis with Double Markers) in Mice. Tasic B, Miyamichi K, Hippenmeyer S, Dani VS, Zeng H, Joo W, Zong H, Chen-Tsai Y, Luo L. PLoS One. 2012;7(3):e33332. Epub 2012 Mar 27. 10.1371/journal.pone.0033332 PubMed 22479386