pBT267_(pCA-ATG-intron-tTA2-TRE-tdT3Myc)
(Plasmid
#36879)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 36879 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript SK(+)
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 3000
- Total vector size (bp) 8161
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nametTA2
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Alt nametetracycline transactivator 2
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Mutationinsertion of a beta-globin intron with a loxP site after start codon of tTA2
- Promoter CAG (chicken beta actin promoter and CMV enhancer)
Cloning Information for Gene/Insert 1
- Cloning method Unknown
- 5′ sequencing primer see sequence
- 3′ sequencing primer see sequence (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nametdT-3Myc
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Alt nametdTomato
- Promoter TRE (tetO)
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Tag
/ Fusion Protein
- 3 Myc tags (C terminal on insert)
Cloning Information for Gene/Insert 2
- Cloning method Unknown
- 5′ sequencing primer see sequence
- 3′ sequencing primer T3 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byW. Hillen
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBT267_(pCA-ATG-intron-tTA2-TRE-tdT3Myc) was a gift from Liqun Luo (Addgene plasmid # 36879 ; http://n2t.net/addgene:36879 ; RRID:Addgene_36879) -
For your References section:
Extensions of MADM (Mosaic Analysis with Double Markers) in Mice. Tasic B, Miyamichi K, Hippenmeyer S, Dani VS, Zeng H, Joo W, Zong H, Chen-Tsai Y, Luo L. PLoS One. 2012;7(3):e33332. Epub 2012 Mar 27. 10.1371/journal.pone.0033332 PubMed 22479386