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pBT255_(pCA-GFP4m)
(Plasmid #36877)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 36877 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript SK(+)
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 5691
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP
  • Alt name
    mut4EGFP
  • Mutation
    F64L; S65T; V163A; S175G
  • Promoter CAG (chicken beta actin promoter and CMV enhancer)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XmaI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer see sequence
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Amy Okada from S. McConnell lab (Okada et al,Imaging cells in the developing nervous system with retrovirus expressing modified green fluorescent protein, 1999, Exp Neurol 156(2): 394-406)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBT255_(pCA-GFP4m) was a gift from Liqun Luo (Addgene plasmid # 36877 ; http://n2t.net/addgene:36877 ; RRID:Addgene_36877)

  • For your References section:

    Extensions of MADM (Mosaic Analysis with Double Markers) in Mice. Tasic B, Miyamichi K, Hippenmeyer S, Dani VS, Zeng H, Joo W, Zong H, Chen-Tsai Y, Luo L. PLoS One. 2012;7(3):e33332. Epub 2012 Mar 27. 10.1371/journal.pone.0033332 PubMed 22479386
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