pBT255_(pCA-GFP4m)
(Plasmid #36877)

• Depositing Lab: Liqun Luo
• Publication: Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Mar 27.

Backbone

• Vector backbone: pBluescript SK(+)
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3000
• Total vector size (bp): 5691
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GFP
• Alt name: mut4EGFP
• Mutation: F64L; S65T; V163A; S175G
• Promoter: CAG (chicken beta actin promoter and CMV enhancer)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XmaI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: see sequence
• 3′ sequencing primer: T3

Resource Information

• Supplemental Documents:
  • pBT255_GenBank_file
• A portion of this plasmid was derived from a plasmid made by: Amy Okada from S. McConnell lab (Okada et al,Imaging cells in the developing nervous system with retrovirus expressing modified green fluorescent protein, 1999, Exp Neurol 156(2): 394-406)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry