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Addgene

pfNL43-dE-EGFP
(Plasmid #36865)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 36865 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC18
  • Backbone size w/o insert (bp) 2686
  • Total vector size (bp) 12880
  • Modifications to backbone
    KasI site was knocked out.
  • Vector type
    Mammalian Expression, Lentiviral, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    XL10 Gold
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    fNL43-dE-EGFP
  • Species
    HIV-I
  • Insert Size (bp)
    10194
  • Mutation
    inserted EGFP after 6350 nucleotide of original NL43, which splits/inactivates viral envelope

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid region from nt9236 to nt308 is derived from pNL43dSL1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pfNL43-dE-EGFP was a gift from Mario Chin (Addgene plasmid # 36865 ; http://n2t.net/addgene:36865 ; RRID:Addgene_36865)
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