Skip to main content
Addgene

Hox B8a
(Plasmid #36862)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 36862 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pGEM-T
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression ; Riboprobe Synthesis

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Hox B8a
  • Alt name
    homeobox B8a
  • Species
    D. rerio (zebrafish)
  • Mutation
    D238E
  • GenBank ID
    30343
  • Entrez Gene
    hoxb8a (a.k.a. fj67g02, gp-8, hoxb8, wu:fj67g02, zgc:64159)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (unknown if destroyed)
  • 3′ cloning site None (unknown if destroyed)
  • 5′ sequencing primer Sp6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that Addgene's sequencing results indicate the Hox B8a insert in this plasmid has a D238E mutation when compared to GenBank entry NP_571195.1. The depositing laboratory believes these are allelic differences common with outbred strains and this is the exact plasmid they used in the associated publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Hox B8a was a gift from Robert Ho (Addgene plasmid # 36862 ; http://n2t.net/addgene:36862 ; RRID:Addgene_36862)

  • For your References section:

    Zebrafish hox genes: genomic organization and modified colinear expression patterns in the trunk. Prince VE, Joly L, Ekker M, Ho RK. Development. 1998 Feb . 125(3):407-20. PubMed 9425136
Related items: