pGEX6P1-Syndapin1fl
(Plasmid
#36859)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 36859 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEX6P1
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Backbone manufacturerGE Healthcare
- Backbone size w/o insert (bp) 4960
- Total vector size (bp) 6292
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSyndapin1
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Alt namePacsin1
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SpeciesM. musculus (mouse)
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Insert Size (bp)1332
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GenBank IDNM_011861
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Entrez GenePacsin1 (a.k.a. A830061D09Rik, H74, mKIAA1379, syndapin)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer pGEX5
- 3′ sequencing primer pGEX3 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe template was purchased from the company BioCat, Heidelberg, Germany.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX6P1-Syndapin1fl was a gift from Yvonne Groemping (Addgene plasmid # 36859 ; http://n2t.net/addgene:36859 ; RRID:Addgene_36859)