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Addgene

Hox D4a
(Plasmid #36469)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 36469 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEM-T
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression ; Riboprobe Synthesis

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Hox D4a
  • Alt name
    homeobox D4a
  • Alt name
    RP71-78H1.3
  • Alt name
    hoxd4
  • Species
    D. rerio (zebrafish)
  • Mutation
    Q100H, A101T, G125C and R189W
  • GenBank ID
    30329
  • Entrez Gene
    hoxd4a (a.k.a. RP71-78H1.3, etID309823.9, etID37000.9, fb36c05, hoxd4, wu:fb36c05)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site None (not destroyed)
  • 5′ sequencing primer Sp6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that Addgene's sequencing results indicate the Hox D4a insert in this plasmid has Q100H, A101T, G125C and R189W mutations when compared to GenBank entry NP_001119917.1. The depositing laboratory believes these are allelic differences common with outbred strains and this is the exact plasmid they used in the associated publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Hox D4a was a gift from Robert Ho (Addgene plasmid # 36469 ; http://n2t.net/addgene:36469 ; RRID:Addgene_36469)

  • For your References section:

    Zebrafish hox genes: genomic organization and modified colinear expression patterns in the trunk. Prince VE, Joly L, Ekker M, Ho RK. Development. 1998 Feb . 125(3):407-20. PubMed 9425136
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