Hox A13b
(Plasmid
#36468)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 36468 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEM-T
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 3000
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Vector typeRiboprobe Synthesis
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namehoxA13b
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Alt namehomeobox A13b
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Alt nameHoxa-13
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Alt namewu:fa66g07
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SpeciesD. rerio (zebrafish)
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MutationF232C
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GenBank IDAAH90761.1
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Entrez Genehoxa11b (a.k.a. Hoxa-11, hoxa11, im:7142641)
- Promoter T7
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Pst (not destroyed)
- 3′ cloning site None (unknown if destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that Addgene's sequencing results indicate the Hox A13b insert in this plasmid contains a F232C mutation when compared to GenBank entry AAH90761.1. The depositing laboratory believes these are allelic differences common with outbred strains and this is the exact plasmid they used in the associated publication.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Hox A13b was a gift from Robert Ho (Addgene plasmid # 36468 ; http://n2t.net/addgene:36468 ; RRID:Addgene_36468) -
For your References section:
Tri-phasic expression of posterior Hox genes during development of pectoral fins in zebrafish: implications for the evolution of vertebrate paired appendages. Ahn D, Ho RK. Dev Biol. 2008 Oct 1;322(1):220-33. Epub 2008 Jul 3. 10.1016/j.ydbio.2008.06.032 PubMed 18638469