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Addgene

Hox D13a
(Plasmid #36467)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 36467 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEM-T
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Riboprobe Synthesis

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    hoxa11b
  • Alt name
    homeobox D13a
  • Alt name
    hoxd13
  • Species
    D. rerio (zebrafish)
  • Mutation
    F63I, P75Q and M133I
  • GenBank ID
    AAI53653.1
  • Entrez Gene
    hoxa11b (a.k.a. Hoxa-11, hoxa11, im:7142641)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site None (unknown if destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that Addgene's sequencing results indicate the Hox D13a insert in this plasmid has F63I, P75Q and M133I mutations when compared to GenBank entry AAI53653.1. The depositing laboratory believes these are allelic differences common with outbred strains and this is the exact plasmid they used in the associated publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Hox D13a was a gift from Robert Ho (Addgene plasmid # 36467 ; http://n2t.net/addgene:36467 ; RRID:Addgene_36467)

  • For your References section:

    Tri-phasic expression of posterior Hox genes during development of pectoral fins in zebrafish: implications for the evolution of vertebrate paired appendages. Ahn D, Ho RK. Dev Biol. 2008 Oct 1;322(1):220-33. Epub 2008 Jul 3. 10.1016/j.ydbio.2008.06.032 PubMed 18638469
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