Hox A13a
(Plasmid #36463)

• Depositing Lab: Robert Ho
• Publication: Ahn et al Dev Biol. 2008 Oct 1;322(1):220-33. Epub 2008 Jul 3.

Backbone

• Vector backbone: pGEM-T
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 3000
• Vector type: Riboprobe Synthesis

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: hoxA13a
• Alt name: homeobox A13a
• Species: D. rerio (zebrafish)
• Mutation: contains aa102-290; M107T
• GenBank ID: 570239
• Entrez Gene: hoxa13a (a.k.a. hoxa13)
• Promoter: T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: None (unknown if destroyed)
• 5′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's sequencing results indicate the Hox A13a insert in this plasmid starts at amino acid 102, contains a M107T mutation and ends at aa290 when compared to GenBank entry NP_001078963.1. The depositing laboratory believes these are allelic differences common with outbred strains and this is the exact plasmid they used in the associated publication.