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Addgene

pNRB143
(Plasmid #36452)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 36452 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    YepFAT4
  • Backbone manufacturer
    K Runge
  • Backbone size w/o insert (bp) 7011
  • Total vector size (bp) 10570
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RAD54
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    3500
  • Entrez Gene
    RAD54 (a.k.a. YGL163C, XRS1)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (not destroyed)
  • 3′ cloning site SacI (destroyed during cloning)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNRB143 was a gift from Douglas Bishop (Addgene plasmid # 36452 ; http://n2t.net/addgene:36452 ; RRID:Addgene_36452)

  • For your References section:

    High copy number suppression of the meiotic arrest caused by a dmc1 mutation: REC114 imposes an early recombination block and RAD54 promotes a DMC1-independent DSB repair pathway. Bishop DK, Nikolski Y, Oshiro J, Chon J, Shinohara M, Chen X. Genes Cells. 1999 Aug;4(8):425-44. 10.1046/j.1365-2443.1999.00273.x PubMed 10526232
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