pMartini Gate A R2-R1
(Plasmid #36428)

• Depositing Lab: Brian McCabe
• Publication: Wang et al PLoS One. 2012;7(7):e42102. Epub 2012 Jul 25.

Backbone

• Vector backbone: pMartini
• Total vector size (bp): 4618
• Modifications to backbone: 5' cloning sites: SfiI-XhoI-HindIII-XbaI-KpnI-Acc65I-SphI-NspI-EcoRV 3' cloning sites: XhoI-SfiI-BanII-SacI-EcoRV
• Vector type: Gateway Conversion Vector

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Gateway Cassette A R2-R1

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13_fwd
• 3′ sequencing primer: M13_rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This vector can be used for conversion of any cloning vector into a gateway destination vector and facilitate the generation of in-frame fusions with a gateway cassette.

A,B or C are reading frames; R1-R2 or R2-R1 indicate the gateway cassette orientation.

Please see the associated publication for more information: Wang J-W, Beck ES, McCabe BD (2012) A Modular Toolset for Recombination Transgenesis and Neurogenetic Analysis of Drosophila. PLoS ONE 7(7): e42102 http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0042102