Bcr del oligo pCDNA3.1/HisB
(Plasmid #36423)

• Depositing Labs: John Groffen, Nora Heisterkamp
• Publication: Yi et al BMC Biochem. 2011 Feb 10;12:8.

Backbone

• Vector backbone: pCDNA3.1/hisB
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Total vector size (bp): 9400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BCR
• Species: H. sapiens (human)
• Mutation: Deletion of amino acid residues 2-63
• Entrez Gene: BCR (a.k.a. ALL, BCR1, CML, D22S11, D22S662, PHL)
• Tag / Fusion Protein:
  • Xpress (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EagI (not destroyed)
• 3′ cloning site: Xba I (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-Rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

To generate Xpress-tagged Bcr del oligo, we
isolated the N-terminal end of Bcr with deletion of amino acid residues 2-63 from a previously described 474 bp subclone by digestion with Eag I x Xho I.The 3.5 kb 3’ end of BCR was isolated from a cDNA clone in pSK (B1/SK) by digestion with Xho I x Xba I.
These two fragments were combined in pcDNA3.1/HisB digested with Not I x Xba.

Construct has a deletion of amino acid residues 2-63. The first amino acid residues are M- AAA- KEKKSY.... The AAA originate from the pSL1180 polylinker, The "KEKK.." are Bcr amino acids. Constructed by Sun-ju Yi.