Bcr del PK
(Plasmid #36422)

• Depositing Labs: John Groffen, Nora Heisterkamp
• Publication: Yi et al J Biol Chem. 2009 Dec 18;284(51):35645-51. Epub .

Backbone

• Vector backbone: pCDE
• Backbone manufacturer: p CD from Paul Berg
• Backbone size w/o insert (bp): 3000
• Total vector size (bp): 6500
• Modifications to backbone: Polylinker from mp13 inserted into the sole cloning site -EcoRI- in the original vector pCD to make pCDE.
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: BCR
• Species: H. sapiens (human)
• Mutation: deletion of residues 40-447
• Entrez Gene: BCR (a.k.a. ALL, BCR1, CML, D22S11, D22S662, PHL)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Construct includes 5' untranslated sequences, and the first 39 aminoacids including a small part of the N-terminal oligomerization domain. The deletion was generated by ligation of a Stu I site in BCR exon 1 to a downstream Nae I site. The construct lacks the ST kinase domain. Vector is a derivative of pCD with a multiple cloning site included. Has SV-40 origin of replication for high yield expression in COS-1 cells. Also see Yi et al 2009. Constructed by Vesa Kaartinen.