EGFP-Abr R683A N795A
(Plasmid #36417)

• Depositing Labs: John Groffen, Nora Heisterkamp
• Publication: Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. Epub 2006 Nov 20.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: active BCR-related
• Alt name: ABR isoform A
• Species: H. sapiens (human)
• Mutation: R683A N795A
• Entrez Gene: ABR (a.k.a. MDB)
• Tag / Fusion Protein:
  • EGFP (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: SV40pA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Mutagenesis was done on EGFP-Abr R683A obtained from EunJoon Kim, KAIST, Korea

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is the ABR isoform A. The R683A N795A double mutant lacks GTPase activity towards RacGTP. Construct: Young-jin Cho. Also see PMID 20962234.