ECFP-Bcr
(Plasmid #36415)

• Depositing Labs: John Groffen, Nora Heisterkamp
• Publication: Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. Epub 2006 Nov 20.

Backbone

• Vector backbone: pECFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 9000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: breakpoint cluster region
• Alt name: BCR
• Species: H. sapiens (human)
• Insert Size (bp): 4300
• Entrez Gene: BCR (a.k.a. ALL, BCR1, CML, D22S11, D22S662, PHL)
• Tag / Fusion Protein:
  • ECFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Bam HI (destroyed during cloning)
• 3′ cloning site: Kpn I (not destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: SV40-pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Constructed by Jess Cunnick. We inserted the BCR part as a 1.7 kb BamHI-Sst I + 2.6 kb Sst I-Kpn I fragment into pECFP-C1 digested with Bgl II x Kpn I in a 3-way ligation.