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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 36413 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBABE-puro
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Backbone manufacturerBob Weinberg (Addgene Plasmid #1764)
- Backbone size w/o insert (bp) 5169
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemTERT
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SpeciesM. musculus (mouse)
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GenBank IDBC127068.1
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Entrez GeneTert (a.k.a. EST2, TCS1, TP2, TR, TRT)
- Promoter LTR
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer mTERT 1: 5′-TTCGAACAGCAAACCAACAG-3′ (Common Sequencing Primers)
Resource Information
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Addgene Notes
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mTert-pBabe-puro was a gift from Marta Alvarez & Joseph Bidwell (Addgene plasmid # 36413 ; http://n2t.net/addgene:36413 ; RRID:Addgene_36413) -
For your References section:
Immortalization and characterization of osteoblast cell lines generated from wild-type and Nmp4-null mouse bone marrow stromal cells using murine telomerase reverse transcriptase (mTERT). Alvarez MB, Childress P, Philip BK, Gerard-O'Riley R, Hanlon M, Herbert BS, Robling AG, Pavalko FM, Bidwell JP. J Cell Physiol. 2012 May;227(5):1873-82. doi: 10.1002/jcp.22915. 10.1002/jcp.22915 PubMed 21732358