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Abr GAP domain R683A N795A/pGEX 3X
(Plasmid #36408)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 36408 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-3X
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Rosetta2
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Abr GAP domain with R683A N795A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    800
  • Mutation
    R683A and N795A
  • Entrez Gene
    ABR (a.k.a. MDB)
  • Tag / Fusion Protein
    • GST (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sma I (destroyed during cloning)
  • 3′ cloning site Eco RI (not destroyed)
  • 5′ sequencing primer pGEX-3X
  • 3′ sequencing primer pGEX-3X
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

We subcloned an 0.8 kb PvuII-EcoRI fragment of human ABR into pGEX-3X digested with Sma I -EcoRI. This encompasses amino acid residues 597-859 of NP_068781. The construct contains the double mutations R683A N795A This double mutation generates a protein without any GTPase activating activity for RacGTP. The construct includes 3' UT sequences of the cDNA. The Pvu II site is located in the amino acid string -QLDP-. Construct made by Young Jin Cho. The Rosetta2 strain may improve yield of the fusion protein.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Abr GAP domain R683A N795A/pGEX 3X was a gift from Nora Heisterkamp (Addgene plasmid # 36408 ; http://n2t.net/addgene:36408 ; RRID:Addgene_36408)

  • For your References section:

    Abr and Bcr, two homologous Rac GTPase-activating proteins, control multiple cellular functions of murine macrophages. Cho YJ, Cunnick JM, Yi SJ, Kaartinen V, Groffen J, Heisterkamp N. Mol Cell Biol. 2007 Feb;27(3):899-911. Epub 2006 Nov 20. 10.1128/MCB.00756-06 PubMed 17116687
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